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mcherry激发波长是多少?

作者:激发光源事业部时间:2019-09-05 10:26:27浏览2700 次

信息摘要:

mCherry是一种来自于蘑菇珊瑚(mushroom coral)的红色荧光蛋白,常有于标记和示踪某些分子和细胞组分。相对于其他荧光,mCherry的好处在于它的颜色和应用最多的绿色荧光蛋白(GFP)能进行共同标记,并且mCherry相对于其他单体荧光蛋白来说也具有卓越的光稳定型。

mcherry激发波长是多少?

 mcherry Fluorescent Proteins Excitation Emission

mCherry是一种来自于蘑菇珊瑚(mushroom coral)的红色荧光蛋白,常有于标记和示踪某些分子和细胞组分。相对于其他荧光,mcherry的好处在于它的颜色和应用最多的绿色荧光蛋白GFP)能进行共同标记,并且mcherry相对于其他单体荧光蛋白来说也具有卓越的光稳定型。

mcherry红色荧光蛋白
吸收和发射波长:

mcherry的更大吸收/发射峰分别位于587nm和610nm,对光致漂白耐受,荧光非常稳定。

mcherry红色荧光蛋白

mcherry常见应用:

•mCherry常用于与目的基因组成融合蛋白以及通过IRES或2A与感兴趣的蛋白共表达;
•启动子活性研究;
•荧光共振能量转移(fluorescence resonance energy transfer,FRET)和其他定量实验;
•标记细胞或者分子,进行示踪实验;

mcherry红色荧光蛋白

 mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria.

 
m前缀的意义:
mCherry的m为monmer单体的缩写,表示mCherry荧光蛋白的形式为单体,这在很多实验设计中非常重要,比如与目的标记基因组成融合蛋白时。

成熟时间:
mCherry具有较快的成熟速度,t0.5为15分钟,这在一些需要做出快速反应的实验非常重要。比如启动子活性报告系统等。

如何观察和筛选mcherry在植物中的表达?

你可以选用LUYOR-3415RG和LUYOR-3260GR便携式荧光蛋白激发光源来直接观察和筛选mcherry在植物中有没有表达。如需进一步了解,请拨打电话153-1756-5658进行咨询(或直接添加微信15317565658咨询)。

mcherry红色荧光蛋白

mcherry红色荧光蛋白的激发波长和发射波长


实验室常见荧光蛋白的激发波长和发射波长


紫外荧光蛋白UV Proteins

ProteinExcitation Wavelength  Emission Wavelength       
Sirius355424
Sandercyanin375630
shBFP-N158S/L173I375458


蓝色荧光蛋白Blue Proteins

ProteinExcitation WavelengthEmission Wavelength
Azurite383450
EBFP2383448
mKalama1385456
mTagBFP2                       399454
TagBFP402457
shBFP401458

青色荧光蛋白Cyan Proteins

ProteinExcitation WavelengthEmission Wavelength
ECFP433475
Cerulean433475
mCerulean3433475
SCFP3A433474
CyPet435477
mTurquoise434474
mTurquoise2434474
TagCFP458480
mTFP1462492
monomeric Midoriishi-Cyan470496
Aquamarine430474

绿色荧光蛋白Green Proteins

ProteinExcitation WavelengthEmission Wavelength
TurboGFP482502
TagGFP2483506
mUKG483499
Superfolder GFP485510
Emerald487509
EGFP488507
Monomeric Azami Green   492505
mWasabi493509
Clover505515
mNeonGreen506517
NowGFP494502
mClover3506518

黄色荧光蛋白Yellow Proteins

ProteinExcitation WavelengthEmission Wavelength
TagYFP508524
EYFP513527
Topaz514527
Venus515528
SYFP2515527
Citrine516529
Ypet517530
lanRFP-ΔS83                      521592
mPapaya1530541
mCyRFP1528594

桔色荧光蛋白Orange Proteins

ProteinExcitation WavelengthEmission Wavelength
Monomeric Kusabira-Orange548559
mOrange548562
mOrange2549565
mKOκ551563
mKO2551565

红色荧光蛋白Red Proteins

ProteinExcitation WavelengthEmission Wavelength
TagRFP555584
TagRFP-T555584
RRvT556583
mRuby558605
mRuby2559600
mTangerine                            568585
mApple568592
mStrawberry574596
FusionRed580608
mCherry587610
mNectarine558578
mRuby3558592
mScarlet569594
mScarlet-I569593

远红荧光蛋白Far Red Proteins

ProteinExcitation WavelengthEmission Wavelength
mKate2588633
HcRed-Tandem590637
mPlum590649
mRaspberry598625
mNeptune600650
NirFP605670
TagRFP657                            611657
TagRFP675598675
mCardinal604659
mStable597633
mMaroon1609657
mGarnet2598671

近红荧光蛋白Near IR Proteins

ProteinExcitation WavelengthEmission Wavelength
iFP1.4684708
iRFP713 (iRFP)                     690713
iRFP670643670
iRFP682663682
iRFP702673702
iRFP720702720
iFP2.0690711
mIFP683704
TDsmURFP642670
miRFP670642670

Sapphire-type Proteins

ProteinExcitation WavelengthEmission Wavelength
Sapphire399511
T-Sapphire                             399511
mAmetrine406

526

Red Fluorescent Proteins

Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.


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