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FITC激发发射波长?

作者:激发光源事业部时间:2019-11-17 08:46:42浏览320 次

信息摘要:

FITC激发发射波长激发波长为488nm,发射波长为525nm,FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.

 

FITC激发发射波长激发波长为488nm,发射波长为525nm

FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.

Fluorescein (FITC) 
Fluorescein derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water solubility.
Fluorescein-based dyes and their conjugates have several performance characteristics that may facilitate or limit use in certain applications. Fluorescein displays:

A relatively high rate of photobleaching
Fluorescent signal that is sensitive to pH changes
A relatively broad fluorescence emission spectrum
Fluorescence quenching on conjugation to biopolymers
 
Excitation max 490nm,Emission max 525nm

FITC激发发射波长

FITC一般指异硫氰酸荧光素,异硫氰酸荧光橙红,异硫氰酸荧光黄,异硫氰酸荧光红,异硫氰酸荧光素异构体I,5-异硫氰酸荧光素。其形态为黄色粉末,有吸湿性,是一种生化试剂,也是医学诊断药物,可对由地细菌病毒和寄生虫等所致疾病进行快速诊断。并且它能与各种抗体蛋白结合,结合后的抗体不丧失与一定抗原结合的特异性,并在碱性溶液中仍有强烈绿色荧光,加酸后析出沉淀,荧光消失,微溶于丙酮、乙醚和石油醚。该品是生化试剂,也是医学诊断药物,主要用于荧光抗体技术中的荧光染料,能和各种抗体蛋白结合,结合后的抗体不丧失与一定抗原结合的特异性,并在碱性溶液中具有强烈的绿色荧光。用于医学,农学和畜牧等方面,可对由地细菌病毒和寄生虫等所致疾病进行快速诊断。

(fluorescein isothiocyanate, FITC) FITC纯品为黄色或橙黄色结晶粉末,易溶于水和酒精溶剂。有两种异构体,其中异构体Ⅰ型在效率、稳定性与蛋白质结合力等方面都更优良。FITC分子量为389.4,更大吸收光波长为490~495nm,更大发射光波长为520~530nm,呈现明亮的黄绿色荧光。FITC在冷暗干燥处可保存多年,是目前应用最广泛的荧光素。其主要优点是人眼对黄绿色较为敏感,通常切片标本中的绿色荧光少于红色。危险代码:Xn,危险等级:R20/21/22-R36/37/38-R42/43。安全等级:S22-S26- S36/37/39-S45。

常见荧光蛋白的激发波长和发射波长:

EBFP2,蓝色荧光蛋白,激发波长383nm,发射波长448nm;
mCelulean,青色荧光蛋白,激发波长433nm,发射波长475nm;
EGFP绿色荧光蛋白,激发波长488nm,发射波长507nm;
EYFP黄色荧光蛋白,激发波长513nm,发射波长527nm;
mCitrine,黄色荧光蛋白,激发波长516nm,发射波长529nm;
mCherry红色荧光蛋白,激发波长587nm,发射波长610nm;
mRuby2,红色荧光蛋白,激发波长559nm,发射波长600nm;
tdTomato,橙色荧光蛋白,激发波长554nm,发射波长581nm;

 


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